PURETIME ® aminopeptidase assays
Key Features Key Benefits
- Enzyme and substrate additions only - One step
- Fluorescent assay - 384 well plate volume or smaller
- Customised peptide sequences - Target enzyme of interest
- Ratiometric or lifetime discrimination - Resists interference
- Instant measurement of aminopeptidase activity - Kinetic measurements easily made
Assay principle
Fluorescence of PURETIME®17 is enhanced upon cleavage, producing more intense emission and a shorter fluorescence lifetime. The small fluorophor does not adversely affect the action of the enzyme. Fluorescence measurements can be made at any point during the aminopeptidase incubation, permitting time course experiments and direct determination of reaction kinetics.
Examples
Aminopeptidase activity assay
Dipeptidyl peptidase IV (DPP-IV) enzyme titration using LA-PURETIME17 substrate (1450nM)
(ratiometric mode above, lifetime mode below)
Aminopeptidase time course assay
Dipeptidyl peptidase IV (DPP-IV) time course using LP-PURETIME17 substrate (1250nM)
(ratiometric mode above, lifetime mode below)
DPP-IV / chymostatin inhibition measurement
Dipeptidyl peptidase IV (DPP-IV) inhibition by serine protease inhibitor chymostatin
using LP-PURETIME17 substrate (1000nM)
Using the lifetime discriminated fluorescence intensity of the product, an IC50 value of 40micM was determined for chymostatin inhibition of dipeptidyl peptidase IV (DPP-IV).
Products
| Product code | N terminus | Sequence | C terminus | Amount |
| PT17-LAP-P | NH2- | LA | -
PURETIME
® 17 |
1mg |
| PT17-DPP-P | NH2- | LP | -
PURETIME
® 17 |
1mg |
| PT17-LEU-P | NH2- | L | -
PURETIME
® 17 |
1mg |
Ordering
We ship direct from the UK all over the world. Please e-mail your request for a quotation or more information to sales@assaymetrics.com. Before placing an order with us, please ensure you have read our Terms and Conditions.