Description:
Bovine Serum Albumin (BSA) is supplied with some restriction enzymes to prevent adhesion of the enzyme to reaction tubes and pipette surfaces. BSA also stabilizes some proteins during incubation. The source of purified BSA is a molecular biology grade, protease free, fraction V starting material. This material is then processed to remove nucleases.
Concentration: 10 mg/ml (or other concentration on request)
Storage Buffer: dest. water or
in NEB-Buffer: 20 mM KPO4, 50 mM NaCl, 0.1 mM EDTA, 5% Glycerol, pH 7.0 (25°C)
Storage Temperature: -20°C
Quality Control:
A 16 hour incubation is performed with ΦX174 DNA- HaeIII Digest. This assay checks for endonuclease, exonuclease and binding protein contamination. 16 hour incubation is performed with ΦX174 RFI DNA, as sensitive assay for nonspecific endonuclease and 1 hour incubation with a RNA ladder is performed. Produced in ISO-certificated Company.
Transport: Ambient or with blue ice